Polymorphisms of COTL1 gene identified by proteomic approach and their association with autoimmune disorders

To select candidate genes, we attempted to comparative analysis of protein levels between rheumatoid arthritis (RA) patients and healthy controls by two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF-MS). We identified 17 proteins that showed up- or down-regulated spots in RA patients. We found that coactosin-like1 (COTL1) were highly expressed in RA patients compared with healthy controls. We performed a case-control study to determine whether the COTL1 gene polymorphisms were associated with RA and systemic lupus erythematosus (SLE). The genotype frequency of c.-1124G>T and the allelic frequency of c.484G>A in RA patients, and the genotype frequency of c.484G>A in SLE patients were significantly different from healthy controls (P = 0.009, 0.027, and 0.025, respectively). We also investigated the correlation with the levels of rheumatoid factor (RF) and anti-cyclic citrullinated peptide (CCP) antibody in RA patients, and anti-nuclear antibodies (ANA) in SLE patients. The c.484G>A polymorphism in RA patients has significant association with the levels of anti-CCP antibody (P = 0.03). Our findings demonstrated that c.-1124G>T and c.484G>A polymorphisms of the COTL1 gene might be associated with the genetic susceptibility of autoimmune disorders.     

Study of proteinous and micellar microenvironment using donor acceptor charge transfer fluorosensor N,N-dimethylaminonaphthyl-(acrylo)-nitrile

Interaction of charge transfer fluorophore N,N-dimethylaminonaphthyl-(acrylo)-nitrile (DMANAN) with globular proteins Human Serum Albumin (HSA) and Bovine Serum Albumin (BSA) brings forth a marked change in the position and intensity of band maxima both in case of absorption and fluorescence spectra. Spectroscopic approach has been elaborately implemented to explore the binding phenomena of the probe with HSA and BSA and it is found that the extent of binding of the probe to both serum albumins is similar in nature. Steady state fluorescence anisotropy values, fluorescence quenching study using acrylamide quencher and Red Edge Excitation Shift (REES) help in drawing reliable conclusions regarding the location of the probe molecule within the hydrophobic cavity of the proteins. An increase in fluorescence lifetime of the probe molecule solubilized in both the proteinous media also indicate that the probe is located at the motionally restricted environment inside the hydrophobic cavity of proteins and hence non-radiative channels are less operative than in the bulk water. Similarly, the variation of position and intensity of the emission maxima of DMANAN solubilized in micellar medium of Sodium Dodecyl Sulphate (SDS) also predicts well the critical micellar concentration (CMC) and polarity of micellar microenvironment.     

Enantiopure inherently chiral calix[4]arene derivatives containing quinolin-2-yl-methanol moiety:Synthesis and application in the catalytic asymmetric addition of diethylzinc to benzaldehyde

A series of novel N,O-type chiral ligands derived from enantiopure inherently chiral calix[4]arenes containing quinolin-2-yl-methanol moiety in the cone or partial-cone conformation have been synthesized and characterized. Moreover, they have been applied to the catalytic asymmetric addition of diethylzinc to benzaldehyde, which represents the first example that the inherently chiral calixarene can be used as the chiral ligands for the catalytic asymmetric synthesis. Supported by the National Natural Science Foundation of China (Grant Nos. 20625206 & 20372064), the National Basic Research Project (Grant Nos. 2007CB808000 & 2008CB617501), and the Chinese Academy of Sciences     

Fabrication of an ��-lipoic acid-eluting poly-(D,L-lactide-co-caprolactone) cuff for the inhibition of neointimal formation

The purpose of this study was to develop a novel polymer cuff for the local delivery of α-lipoic acid (ALA) to inhibit neointimal formation in vivo. The polymer cuff was fabricated by incorporating the ALA into poly-(_D,L-lactide-co-caprolactone) 40:60 (PLC), with or without methoxy polyethylene glycol (MethoxyPEG). The release kinetics of ALA and in vitro degradation by hydrolysis were analyzed by HPLC and field emission scanning electron microscopy (FE-SEM), respectively. In vivo evaluation of the effect of the ALA-containing polymer cuff was carried out using a rat femoral artery cuff injury model. At 24 h, 48% or 87% of the ALA was released from PCL cuffs with or without MethoxyPEG. FE-SEM results indicated that ALA was blended homogenously in the PLC with MethoxyPEG, whereas ALA was distributed on the surface of the PLC cuff without MethoxyPEG. The PLC cuff with MethoxyPEG showed prolonged and controlled release of ALA in PBS, in contrast to the PLC cuff without MethoxyPEG. Both ALA-containing polymer cuffs had a significant effect on the inhibition of neointimal formation in rat femoral artery. Novel ALA-containing polymer cuffs made of PLC were found to be biocompatible and effective in inhibiting neointimal formation in vivo. Polymer cuffs containing MethoxyPEG allowed the release of ALA for one additional week, and the rate of drug release from the PLC could be controlled by changing the composition of the polymer. These findings demonstrate that polymer cuffs may be an easy tool for the evaluation of anti-restenotic agents in animal models.     

Phase composition and phase transformation in Bi(Sb,Nb,Ta)O4 system

In this work Bi(Sb_xNb_yTa_z)O₄ (x + y + z = 1) samples are prepared using mixed-oxide method. A pseudo-ternary phase diagram of Bi(Sb,Nb,Ta)O₄ system is given below the melting point. It is composed of a monoclinic phase region, an orthorhombic phase region and a monoclinic–orthorhombic co-existing phase region. In the orthorhombic phase region, the transformation from orthorhombic to triclinic phase is found to be sensitive to the composition and sintering temperature. Both the transformation from monoclinic to orthorhombic structure and the transformation from orthorhombic to triclinic structure have been studied by the cell parameters.     

抽象度量空间上的一致连续函数空间

本文主要讨论抽象度量空间上的一致连续函数空间的Banach空间结构,代数结构和格结构．     

Weighted composition operators from F（p,q, s） spaces to Bers-type spaces in the unit ball

This paper deals with the boundedness and compactness of the weighted composition operators from the F（p, q, s） spaces, including Hardy space, Bergman space, Qp space, BMOA space, Besov space and α-Bloch space, to Bers-type spaces Hv^∞（ or little Bers-type spaces Hv,o∞ ）, where v is normal.     

具有Monod-Haldane功能反应和脉冲效应的捕食系统的动力学性质

基于综合害虫防治,对具脉冲效应的Monod—Haldane功能反应的捕食系统进行了分析,根据Floquet乘子理论,获得了害虫灭绝周期解全局渐近稳定与系统持续生存的条件．并讨论了害虫灭绝周期解附近分支出非平凡周期解的问题,且文章利用Matlab软件对害虫灭绝周期解害虫周期爆发现象进行了数值模拟．     

不完备信息下的灰色粗糙集成决策模型侯亚林

In this paper, for multiple attribute decision-making problem in which attribute values are interval grey numbers and some of them are null values, a decision model based on grey rough sets integration with incomplete information is proposed. We put forward incidence degree coefficient formula for grey interval, by information entropy theory and analysis technique, the method and principle is presented to fill up null values. We also establish the method of grey interval incidence cluster. Because grey system theory and Rough set theory are complementary each other, decision table with preference information is obtained by the result of grey incidence cluster. An algorithm for inducing decision rules based on rough set theory and the dominance relationship is presented. In some extent, this algorithm can deal with decision-making problem in which the attribute values are interval grey numbers and some of them are null values. Contrasted with classical model of cluster decision-making, the algorithm has an advantage of flexibility and compatibility to new information.     

多变量整体模式累加多层统计模型的建立及其在组织绩效预测上的应用研究

为了解决多层的少样本或无规则数据的建模问题,在一般多层统计模型的基础上提出了多变量整体模式的累加多层统计模型。此模型把累加方法的优点(将无规则数据转化成有规则数据)与多层统计模型结合起来,拓展了多层统计模型的适用范围。从其在香蕉组织绩效的分析以及在仅有两个调查数据香蕉组织形式绩效的预测中,可以看出此模型有较强的实用性。